Comparison of SUPG and FVG techniques on KSR-1
نویسنده
چکیده
منابع مشابه
Enhancement in colonization of bovine spermatogonial stem cells following addition of knock-out serum replacement to culture medium
Enrichment of cell suspension with germ cells prior to injection into recipient seminiferous tubules is of importance in spermatogonial stem cells (SSCs) transplantation. Knock-out serum replacement (KSR) has been reported to enhance the proliferation of murine SSCs and human embryonic stem cells. The aim of the present study was to investigate the effect of KSR versus fetal bovine serum (FBS) ...
متن کاملThe protein kinase KSR interacts with 14-3-3 protein and Raf
BACKGROUND KSR (kinase suppressor of Ras) is a recently identified putative protein kinase that positively mediates the Ras signaling pathway in the invertebrates Caenorhabditis elegans and Drosophila melanogaster. The function of vertebrate KSR is not well characterized biochemically or biologically. RESULTS We examined the physiological role of KSR in vertebrate signal transduction using Xe...
متن کاملStabilized finite element methods to simulate the conductances of ion channels
We have previously developed a finite element simulator, ichannel, to simulate ion transport through three-dimensional ion channel systems via solving the Poisson–Nernst–Planck equations (PNP) and Sizemodified Poisson–Nernst–Planck equations (SMPNP), and succeeded in simulating some ion channel systems. However, the iterative solution between the coupled Poisson equation and the Nernst–Planck e...
متن کاملRas signaling: PP2A puts Ksr and Raf in the right place
Cells are exposed to a vast array of extracellular stimuli that they have to interpret in a cell-type specific manner. In metazoans, at least five distinct mitogenactivated protein kinase (MAPK) signaling cascades have evolved that couple extracellular signals to a variety of cellular outputs. The core structure of each MAPK signaling pathway is a module of three kinases (MAPK kinase kinase (MA...
متن کاملKinase Suppressor of Ras Signals through Thr of c-Raf-1*
We recently established a two-stage in vitro assay for KSR kinase activity in which KSR never comes in contact with any recombinant kinase other than c-Raf-1 and defined the epidermal growth factor (EGF) as a potent activator of KSR kinase activity (Xing, H. R., Lozano, J., and Kolesnick, R. (2000) J. Biol. Chem. 275, 17276–17280). That study, however, did not address the mechanism of c-Raf-1 s...
متن کامل